DB細(xì)胞
ATCC Number： CRL-2289?
相關(guān)**： 其他**
細(xì)胞類型： B**細(xì)胞
細(xì)胞形態(tài)： **樣
是否是腫瘤細(xì)胞： 1
物種來源： 人
**類型： IgG; lambda light chain
年限： 45 years
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
DB細(xì)胞Designations: DB
Depositors: M Beckwith
Isotype: IgG; lambda light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: large cell lymphoma
Cell Type: B lymphoblast;
Cellular Products: immunoglobulin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. DB細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: CD19 +; CD20 +; CD22 +; Hle-1 +; CD21 -; CD25 -
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 11
THO1: 6,8
TPOX: 8
vWA: 15,16
Age: 45 years
Gender: male
Ethnicity: Caucasian
Comments: The DB cell line was established by Walter J. Urba and Dan L. Longo from ascites fluid from a patient with a diffuse large cell lymphoma.
DB細(xì)胞The cells do not express HLA-DR, HLA-DQ, IgM , IgD or T cell rantigen receptor.
Exposure of DB cells to phorbol esters (PMA or PdBu) that activate protein kinase C results in profound growth inhibition.
They are reported to be negative for the Epstein-Barr virus genome.
The cells were cured of a prior mycoplasma contamination by a 21 day treatment with BM-cycline.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 to 8 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22555: Beckwith M, et al. DB細(xì)胞Phorbol ester-induced, cell-cycle-specific, growth inhibition of human B-lymphoma cell lines. J. Natl. Cancer Inst. 82: 501-509, 1990. PubMed: 2313723
