Tera-2細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 1
物種來源： 人
細(xì)胞形態(tài)： 上皮樣
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
ATCC Number： HTB-106?
相關(guān)**： 其他**
年限： 22 years
Designations: Tera-2
Depositors: J Fogh
Tera-2細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Disease: malignant embryonal carcinoma
Derived from metastatic site: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.
Antigen Expression: Tera-2細(xì)胞Blood Type A; Rh+
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 11,12,13
D5S818: 9,12
D7S820: 10,12
THO1: 9.3
TPOX: 8
vWA: 18,19
Cytogenetic Analysis: (P13) hypotriploid (+A2, +A3, +B, +C, +E, +F, -A1) with abnormalities including acrocentric fragmentation and secondary constrictions
Isoenzymes: AK-1, 1
ES-D, 2
G6PD, B
GLO-I, 1
Me-2, 2
PGM1, 1
PGM3, 1
Tera-2細(xì)胞Age: 22 years
Gender: male
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
Subculturing: Protocol: Subcultures are prepared by scraping. Remove spent medium, add fresh, dislodge cells from the floor of the flask, aspirate to disperse and dispense into new flasks. Subculture about once a week.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
References: 22536: Fogh J, et al. Tera-2細(xì)胞Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
