Calu-1細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

年限： grade III

生長狀態(tài)： 貼壁生長

ATCC Number： HTB-54?

相關(guān)**： 上皮細(xì)胞癌

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來源： 人

器官來源： 肺

Designations: Calu-1

Depositors: J Fogh

Calu-1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Tumor Stage: grade III

Disease: epidermoid carcinoma

Derived from metastatic site: pleura

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) Calu-1細(xì)胞The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Applications: transfection host (Roche Transfection Reagents)

Tumorigenic: Yes

Antigen Expression: Blood Type A; Rh+; HLA A10, A11, B15, Bw35

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 11,12

D16S539: 11

D5S818: 10,12

D7S820: 9,10

THO1: 9,9.3

TPOX: 8

vWA: 15,16

Cytogenetic Analysis: Calu-1細(xì)胞The stemline chromosome number is hypotriploid and the 2S component occurred at 14.2%. Modal chromosome number is 62. Seven markers occurred frequently, M1 (two copies per cell), M6 and M7 were found in most cells; M2 and M3, and M4 and M5 appeared to be mutually exclusive, i.e., only one of M2 or M3, and one of M4 or M5 were present in each cell. A Y chromosome was not detected by QM band examination, although the cell line was initiated from a male.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1-2

PGM1, 1-2

PGM3, 1

Age: 47 years

Gender: male

Ethnicity: Caucasian

Comments: Ultrastructural features include numerous microvilli, prominent RER, lysosomes, lipid inclusions, no virus particles.

Contains the ras (K-ras) oncogene.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Interval: every 6 to 8 days

Subcultivation Ratio: Calu-1細(xì)胞A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: recommended serum:ATCC 30-2020

References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23220: Tew KD, Taylor DM. Studies with cyclophosphamide labeled with phosphorus-32: nucleic acid alkylation and its effect on DNA synthesis in rat tumor and normal tissues. J. Natl. Cancer Inst. 58: 1413-1418, 1977. PubMed: 857031

24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047