SNU-C2A細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

年限： 43 years

生長(zhǎng)狀態(tài)： 貼壁，多細(xì)胞聚集

ATCC Number： CCL-250.1?

相關(guān)**： 大腸癌

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

器官來(lái)源： 盲腸

細(xì)胞形態(tài)： 上皮樣

Designations: SNU-C2A

Depositors: AF Gazdar

SNU-C2A細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: loosely adherent, multicell aggregates

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cecum

Disease: colorectal carcinoma

Cellular Products: carcinoembryonic antigen (CEA), 5 ng/ml per 10 exp6 cells per 10 days; TAG-72 surface antigen

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Tumorigenic: Yes

Antigen Expression: Blood type A; Rh+

Cytogenetic Analysis: SNU-C2A細(xì)胞modal number = 46; range = 44 to 48.

This is a pseudodiploid human cell line. The modal chromosome number is 46, occurring at 86%, with polyploidy at 16.2%. The ins(1;?)(p13;?), del(3)(p23), t(4q,?), t(8;?), del(9)(p13), del(11)(p13), 14q+ and two other marker chromosomes are common to all metaphases examined. Other altered chromosomes, if any, occur only sporadically. Normal N11 is absent. Two copies of X chromosomes are found in each cell. The Y chromosome is not found in QM stained preparations.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 2

PGM1, 1

PGM3, 1

Age: 43 years

Gender: female

Ethnicity: Asian

Comments: This is one of 14 colorectal carcinoma cell lines derived by J.G. Park and associates during the years 1982 through 1985.

The line was obtained from a third passage nude mouse xenograft of tumor fragments obtained from a patient prior to therapy.

SNU-C2A細(xì)胞The line was initially grown in RPMI 1640 medium with 20% fetal bovine serum, but was later adapted to growth in the chemically defined medium ACL-4 (it can also be grown in medium with 10% serum).

The cells and tumors formed from the cells produce detectable amounts of Dopa decarboxylase and the TAG-72 antigen.

Propagation: ATCC complete growth medium: ACL-4 medium supplemented with 10% FBS

The base medium for this cell line is ATCC-formulated DMEM: F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:

0.02 mg/ml insulin

0.01 mg/ml transferrin

25 nM sodium selenite

50 nM Hydrocortisone

1 ng/ml Epidermal Growth Factor (do not filter)

0.01 mM ethanolamine

0.01 mM phosphorylethanolamine

100 pM triiodothyronine

0.5% (w/v) bovine serum albumin

10 mM HEPES

0.5 mM sodium pyruvate

2mM L-glutamine(for final conc. of 4.5 mM)

10% FBS

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: Growth is slow.

Subculturing: Protocol: Cultures can be maintained by scraping off the attached cells and transfer along with the the floating cells into new flasks.

Medium Renewal: Two times weekly

Preservation: Freeze medium: SNU-C2A細(xì)胞Complete growth medium supplemented with an additonal 10% fetal bovine serum and 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249