U-2 OS細(xì)胞

ATCC Number： HTB-96?

相關(guān)**： 骨肉瘤

數(shù)量： 大量

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

器官來(lái)源： 骨

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

年限： 15 years

細(xì)胞形態(tài)： 上皮樣

Designations: U-2 OS

Depositors: Hellstrom

U-2 OS細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: bone

Disease: osteosarcoma

Cellular Products: osteosacoma derived growth factor (ODGF)

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Applications: transfection host

Receptors: insulin-like growth factor I (IGF-I); insulin-like growth factor II (IGF II)

Antigen Expression: Blood Type A; Rh+; HLA A2, Aw30, B12, Bw35, B40(+/-)

DNA Profile (STR): U-2 OS細(xì)胞Amelogenin: X

CSF1PO: 13

D13S317: 13

D16S539: 11,12

D5S818: 11

D7S820: 11,12

THO1: 6,9.3

TPOX: 11,12

vWA: 14,18

Cytogenetic Analysis: Cell line U-2 OS is chromosomally highly altered, with chromosome counts in the hypertriploid range. We did not find the hypodiploid cell population described by J. Ponten, et al., Instead, most of the population has slightly higher counts than first described. Very few normal chromosomes are present, but a high number of stable marker chromosomes are identified., Different chromosomal rearrangements involving the same chromosomes (N1, N7, N9, and N11 particularly), are seen. Twenty-two markers are found including: t(9qter--->9q21::1p36--->1p::), 7p+, iso(17q), t(15q;?), 4q+, del(3)(q21), 5q(aberrant) and others. [22509]

U-2 OS細(xì)胞Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 2

PGM3, 1

Age: 15 years

Gender: female

Ethnicity: Caucasian

Comments: J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma of the tibia of a 15 year old girl.

Viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses.

Mycoplasma contamination was detected and eliminated in 1972.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: U-2 OS細(xì)胞2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 22237: Heldin CH, et al. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A-chains. Nature 319: 511-514, 1986. PubMed: 3456080

22509: Ponten J, Saksela E. Two established in vitro cell lines from human mesenchymal tumours. Int. J. Cancer 2: 434-447, 1967. PubMed: 6081590

23011: Raile K, et al. Human osteosarcoma (U-2 OS) cells express both insulin-like growth factor-I (IGF-I) receptors and insulin-like growth factor-II/mannose-6- phosphate (IGF-II/M6P) receptors and synthesize IGF-II: autocrine growth stimulation by IGF-II via the IGF-I receptor. J. Cell. Physiol. 159: 531-541, 1994. PubMed: 8188767

32288: Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

32308: Moradpour D, et al. Characterization of cell lines allowing titghtly regulated expression of heapatitis C virus core protein. Virology 222: 51-63, 1996. PubMed: 8806487