769-P細胞

ATCC Number： CRL-1933?

器官來源： 腎臟

相關(guān)**： 其他**

數(shù)量： 大量

細胞形態(tài)： 上皮樣

運輸方式： 凍存運輸

年限： 63 years

生長狀態(tài)： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

Designations: 769-P

Depositors: RD Williams

769-P細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: kidney

Disease: renal cell adenocarcinoma

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Isolation: Isolation date: 1975

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 10,14

D16S539: 9,13

D5S818: 12

D7S820: 10,11

THO1: 6,9.3

TPOX: 8,11

vWA: 18

Cytogenetic Analysis: 769-P細胞This human cell line contained large numbers of tetra-, hexa-, and higher-ploid cells (2s populations). The modal (s) cell population which occurred in 32% of cells had the pseudodiploid karyotype, 46,XX,-3,-18,del(7) (q21.12;q22.3), ?t(3q?18q). The rate of 2s cells was 42%. Two marker chromosomes, del(7) (q21.12;q22.3) and ?t(3q?18q), were present in all s metaphases, but ?t(3q?18q) was absent in 2s cells. DMs were found in some s cells whereas they were seen in the majority of 2s cells. HSR chromosomes were not found. In s metaphases, both N3 and N18 had only single copy, and the X chromosome was paired.

Age: 63 years

Gender: female

Ethnicity: Caucasian

Comments: This line was derived from a primary clear cell adenocarcinoma.

The cells are globular with indistinct borders, have a high nucleus to cytoplasm ratio and exhibit both microvilli and desmosomes.

They can be grown in soft agar.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

769-P細胞Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 35 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22648: Williams RD, et al. In vitro cultivation of human renal cell cancer. I. Establishment of cells in culture. In Vitro 12: 623-627, 1976. PubMed: 1010528

22653: Williams RD, et al. In vitro cultivation of human renal cell cancer. II. 769-P細胞Characterization of cell lines. In Vitro 14: 779-786, 1978. PubMed: 721102