LS1034細(xì)胞

器官來(lái)源： 盲腸

年限： Dukes' type C

ATCC Number： CRL-2158?

相關(guān)**： 大腸癌

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

Designations: LS1034

Depositors: L Suardet

LS1034細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: cecum

Tumor Stage: Dukes' type C

Disease: colorectal carcinoma

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Isolation: Isolation date: 1989

Tumorigenic: Yes

Oncogene: p53 + (mutated, Gly --> Ser mutation at position 245), APC (mutated, deletion, GAAAAGATT --> GATT at codon 1309)

Antigen Expression: carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive

DNA Profile (STR): LS1034細(xì)胞Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 12

D16S539: 9,11

D5S818: 12,13

D7S820: 8,12

THO1: 7

TPOX: 9,11

vWA: 17

Cytogenetic Analysis: modal number = 77, YY; seven markers involving chromosomes 1, 7, 14, X, and 18 were recognized. The other chromosomes involved could not be properly identified.; Monosomy 7 and monosomy 14 were present in all 15 metaphases analyzed. No normal chromosomes 5 or 18 were observed.

Age: 54 years

Gender: male

Ethnicity: Caucasian

Comments: LS1034 is a colorectal carcinoma cell line isolated in 1989 from a primary tumor biopsy from a 54-year old Caucasian male patient diagnosed with Dukes' Type C, moderately to poorly differentiated cecal carcinoma.

LS1034細(xì)胞More than 90% of LS1034 cells express surface CEA.

The cells express the major histocompatibility (MHC) class I antigens and beta 2 microglobulin, but class II antigens, (HLA-DR, DQ, and DP) were not detected.

No measurable amount of latent transforming growth factor beta-1 (TGF beta-1) is secreted.

Picomolar concentrations of TGF beta-1, TGF beta-2, and TGF beta-3 inhibit the proliferation of LS1034 cells.

LS1034 cells are multidrug resistant (MDR), and can proliferate in serum free medium.

The patient did not exhibit familial adenomatous polyposis (FAP) and normal tissue did not have the deletion in the APC gene.

The colony forming efficiency was 3% in methylcellulose medium containing 5% fetal bovine serum, and the clonogenicity is increased 600% in serum free methylcellulose as compared to semisolid medium containing serum.

A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and was cured by a 21 day treatment with BM Cycline.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended

Medium Renewal: LS1034細(xì)胞Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 33 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23099: Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643

23339: Burmester JK, et al. Characterization of distinct functional domains of transforming growth factor beta. Proc. Natl. Acad. Sci. USA 90: 8628-8632, 1993. PubMed: 7690965

23418: Li CY, et al. Potential role of WAF1/Cip1/p21 as a mediator of TGF-beta cytoinhibitory effect. J. Biol. Chem. 270: 4971-4974, 1995. PubMed: 7890601

26172: Cottrell S, et al. Molecular analysis of APC mutations in familial adenomatous polyposis and sporadic colon carcinomas. Lancet 340: 626-630, 1992. PubMed: 1355210