SK-ES-1細(xì)胞

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

年限： 18 years

ATCC Number： HTB-86?

器官來(lái)源： 骨

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

Designations: SK-ES-1

Depositors: G Trempe, LJ Old

SK-ES-1細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: bone

Disease: sarcoma (anaplastic osteosarcoma or Ewing's sarcoma)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Isolation: Isolation date: 1971

Tumorigenic: Yes

Antigen Expression: Blood Type A; Rh+

DNA Profile (STR): SK-ES-1細(xì)胞Amelogenin: X,Y

CSF1PO: 11

D13S317: 8,9

D16S539: 11

D5S818: 12

D7S820: 10,11

THO1: 6,9.3

TPOX: 8

vWA: 14,17

Cytogenetic Analysis: modal number = 49; range = 44 to 51. This is a hyperdiploid human cell line with the modal chromosome number of 49 occurring in 34% of cells. However, cells with 50 chromosomes also occurred at high percentage (24%). At least 11 markers were consistently present in most cells. Among them were: der(3)t(3;12) (q21;q13); del(3) (q21); inv(1) (p36.1q21), and der(16)t(5;16) (q13;p13.3), all present in single copy per cell. At least three Y-autosome markers were detected. Single normal X chromosome was found in every cell. Normal Y chromosome was not found.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 1-2

Age: 18 years

SK-ES-1細(xì)胞Gender: male

Ethnicity: Caucasian

Comments: Antibodies to the sarcoma lines were detected in sera of patients with sarcomas after absorption with non-sarcoma lines. Sera from normal donors were also cytotoxic.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.

22423: Bloom ET. Further definition by cytotoxicity tests of cell surface antigens of human sarcomas in culture. Cancer Res. 32: 960-967, 1972. PubMed: 4502173

22536: Fogh J, et al.SK-ES-1細(xì)胞 Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871