SW 982細胞

運輸方式： 凍存運輸

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： HTB-93?

相關(guān)**： 其他**

年限： 25 years

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

細胞形態(tài)： 混合型

組織來源： synovium

Designations: SW 982 [SW-982, SW982]

SW 982細胞Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: mixed

Source: Tissue: synovium

Disease: synovial sarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 12,13

D16S539: 11,12

D5S818: 11,13

D7S820: 9,11

THO1: 9.3

SW 982細胞TPOX: 9,11

vWA: 19,20

Cytogenetic Analysis: Hyperdiploid; modal number = 48; range = 42 to 58. The rate of higher ploidies was 1.6%. Nine markers were common to all cells. These were: t(1q4p), del(5) (q31;q33), der(9) t(4;9) (q11;p24), t(8q12p), t(9q13q) and four others.Double minutes (DM) were seen in some cells (usually only one copy). Normal N9 was absent, N4, N8, and N13 were consistently single-copied and the X was double-copied.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1-2

PGM3, 1-2

Age: 25 years

Gender: female

Ethnicity: Caucasian

Comments: The SW 982 cell line was initiated by A. Leibovitz in 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen of a biphasic synovial sarcoma removed from a 25 year old female Caucasian.

The histopathology evaluation reported an undifferentiated malignant tumor consistent with liposarcoma.

An ampule at passage 4 was received at the ATCC in January, 1982.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. SW 982細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

References: 22536: Fogh J, et al. SW 982細胞Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871