SW 1573細胞
運輸方式： 凍存運輸
ATCC Number： CRL-2170?
相關(guān)**： 其他**
是否是腫瘤細胞： 1
物種來源： 人
器官來源： 肺
數(shù)量： 大量
年限： 44 years
生長狀態(tài)： 貼壁生長
細胞形態(tài)： 上皮樣
Designations: SW 1573 [SW-1573, SW1573]
Depositors: W McCombs
SW 1573細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Disease: alveolar cell carcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: These cells are distributed for research purposes only. The Scott and White Clinic releases the line subject to the following: 1) The cells or products derived from them must not be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. SW 1573細胞Commercial interests are the exclusive property of the Scott and White Clinic. 2) Any proposed commercial use of these cells or products produced by them must first be negotiated with the Scott and White Clinic, 2401 S. 31 Street, Temple, Texas 76508. Telephone (817) 774-2432
Antigen Expression: Blood Type O; Rh +
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 12,14
D5S818: 12,13
D7S820: 9,11
THO1: 6,9.3
TPOX: 8,11
vWA: 20
Age: 44 years
Gender: female
Ethnicity: Caucasian
SW 1573細胞Comments: The SW 1573 line was established in 1977 by A. Leibovitz from an alveolar cell carcinoma.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 100%
Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml), and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 22551: Wright WC, et al. SW 1573細胞Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis. J. Natl. Cancer Inst. 66: 239-247, 1981. PubMed: 6935474
