H9/HTLV-IIIB細胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

ATCC Number： CRL-8543?

細胞類型： T**細胞

是否是腫瘤細胞： 1

物種來源： 人

運輸方式： 凍存運輸

細胞形態(tài)： **樣

Designations: H9/HTLV-IIIB

H9/HTLV-IIIB細胞Depositors: The United States of America

Biosafety Level: 2 [Cells Contain RETROVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Cell Type: T lymphocyte;

Cellular Products: Human T cell leukemia virus III (HTLV III); Human Immunodeficiency virus (HIV)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: H9/HTLV-IIIB細胞The cells produce HTLV III.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

H9/HTLV-IIIB細胞Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3536: Popovic M, et al. Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions. US Patent 4,520,113 dated May 28 1985

22938: Chang SY, et al. The origin of HIV-1 isolate HTLV-IIIB. Nature 363: 466-469, 1993. PubMed: 8502298

32754: Trkola A, et al. Human momoclonal antibody 2G12 defines a distinctive neutralization epitope on the gp120 glycoprotein of human immunodeficiency virus type 1. J. Virol. 70: 1100-1108, 1996. H9/HTLV-IIIB細胞PubMed: 8551569