C32TG細(xì)胞
生長狀態(tài)： 貼壁生長
運(yùn)輸方式： 凍存運(yùn)輸
器官來源： 皮膚
數(shù)量： 大量
是否是腫瘤細(xì)胞： 1
物種來源： 人
ATCC Number： CRL-1579?
相關(guān)**： 其他**
年限： 53 *****
Designations: C32TG [C32-r16TG]
C32TG細(xì)胞Depositors: TR Chen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology:
Source: Organ: skin
Disease: melanoma, amelanotic
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Tumorigenic: Yes
DNA Profile (STR): C32TG細(xì)胞Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 11,12
D5S818: 12
D7S820: 9,11
THO1: 9.3
TPOX: 9
vWA: 14,19
Age: 53 *****
Gender: male
C32TG細(xì)胞Ethnicity: Caucasian
Comments: The cells are resistant to 6-thioguanine and sensitive to HAT medium.
This line was derived from C32 (ATCC CRL-1585) by selection for 6-thioquanune resistance.
They are deficient in hypoxanthine-guanine phosphoribosyl transferase (HGPRT-, HPRT-).
Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids, 90%; fetal bovine serum, 10%; 0.050 mM 6-thioguanine
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution several minutes until cells detach. Add fresh medium and centrifuge. Resuspend in fresh medium and dispense into new flasks.
Related Products: derivative:ATCC CRL-1585
References: 1082: Chen TR. Chromosome changes in 6-TG-resistant mutant strains derived from a karyotypically stable human line, C32. Cytogenet. Cell Genet. 35: 181-189, 1983. PubMed: 6861522
26091: Chen TR. Evolution in vitro of stemlines with minimal karyotypic deviations in a human heteroploid cell line. J. Natl. Cancer Inst. 61: 277-284, 1978. PubMed: 277716
26092: Chen TR, Shaw MW. C32TG細(xì)胞Stable chromosome changes in human malignant melanoma. Cancer Res. 33: 2042-2047, 1973. PubMed: 4725368
