TE 130.T細(xì)胞

ATCC Number： CRL-7746?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 成纖維樣

年限： 6 months

是否是腫瘤細(xì)胞： 1

物種來源： 人

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來源： 骨

Designations: TE 130.T

TE 130.T細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast-like

Source: Organ: bone

Disease: sacrococcygeal teratoma

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Age: 6 months

Gender: male

Ethnicity: Caucasian

Comments: TE 130.T細(xì)胞Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

TE 130.T細(xì)胞Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:TE 130.T細(xì)胞ATCC 30-2020