LNCaP clone FGC細(xì)胞

ATCC Number： CRL-1740?

相關(guān)**： 腫瘤

是否是腫瘤細(xì)胞： 1

物種來源： 人

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 前列腺

年限： 50 years *****

數(shù)量： 大量

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

Designations: LNCaP clone FGC

Depositors: JS Horoszewicz

LNCaP clone FGC細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, single cells and loosely attached clusters

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: prostate

Disease: carcinoma

Derived from metastatic site: left supraclavicular lymph node

Cellular Products: human prostatic acid phosphatase; prostate specific antigen

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Isolation: LNCaP clone FGC細(xì)胞Isolation date: 1977

Applications: transfection host

Receptors: androgen receptor, positive; estrogen receptor, positive [23045]

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 10,12

D16S539: 11

D5S818: 11,12

D7S820: 9.1,10.3

THO1: 9

TPOX: 8,9

vWA: 16,18

Cytogenetic Analysis: This is a hypotetraploid human cell line. The modal chromosome number was 84, occurring in 22% of cells. However, cells with chromosome counts of 86 (20%) and 87 (18%) also occurred at high frequencies. The rate of cells with higher ploidies was 6.0%.

Age: 50 years *****

Gender: male

Ethnicity: Caucasian

Comments: LNCaP clone FGC細(xì)胞LNCaP clone FGC was isolated in 1977 by J.S. Horoszewicz, et al., from a needle aspiration biopsy of the left supraclavicular lymph node of a 50-year-old Caucasian male (blood type B+) with confirmed diagnosis of metastatic prostate carcinoma.

These cells are responsive to 5-alpha-dihydrotestosterone (growth modulation and acid phosphatase production).

The cells do not produce a uniform monolayer, but grow in clusters which should be broken apart by repeated pipetting when subcultures are prepared.

They attach only lightly to the substrate, do not become confluent and rapidly acidify the medium.

Growth is very slow.

The cells should be allowed to incubate undisturbed for the first 48 hours after subculture.

When flask cultures are shipped, the majority of the cells become detached from the flask and float in the medium.

Upon receipt, incubate the flask (in the usual position for monolayer cultures) for 24 to 48 hours to allow the cells to re-attach.

The medium can then be removed and replaced with fresh medium.

If desired, the contents of the flask can be collected, centrifuged at 300 X g for 15 minutes, resuspended in 10 ml of medium and dispensed into a single flask.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

LNCaP clone FGC細(xì)胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Maintain cultures at a cell concentration between 1 X 10(4) and 2 X 10(5) cells/cm2.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: about 34 hours

Related Products: LNCaP clone FGC細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

derivative:ATCC CRL-10995

purified DNA:ATCC CRL-1740D

References: 21889: . Models for prostate cancer. 37New York: Liss; 1980.

22410: Gibas Z, et al. A high-resolution study of chromosome changes in a human prostatic carcinoma cell line (LNCaP). Cancer Genet. Cytogenet. 11: 399-404, 1984. PubMed: 6584201

23045: Horoszewicz JS, et al. LNCaP model of human prostatic carcinoma. Cancer Res. 43: 1809-1818, 1983. PubMed: 6831420

32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

33090: Boffa LC, et al. Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence. J. Biol. Chem. 271: 13228-13233, 1996. PubMed: 8662737