Hs 505.T細(xì)胞
器官來源： **結(jié)
是否是腫瘤細(xì)胞： 1
物種來源： 人
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
ATCC Number： CRL-7306?
相關(guān)**： 其他**
細(xì)胞形態(tài)： 成纖維樣
數(shù)量： 大量
Designations: Hs 505.T
Depositors: Naval Biosciences Laboratory
Hs 505.T細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: lymph node
Disease: chronic lymphocytic leukemia (CLL)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 10,13
D5S818: 12
D7S820: 8,10
THO1: 8,9
TPOX: 6,8
vWA: 15
Hs 505.T細(xì)胞Gender: female
Ethnicity: Black
Comments: Patient had Kaposi's sarcoma.
Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:Volumes used in this protocol are for 75 sq. cm. flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: Hs 505.T細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37.0°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer cell suspension to a centrifuge tube and centrifuge at approximately 125 xg for 5 to 10 minutes. Discard supernatant.
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37.0°C.
Subcultivation ratio: 1:2 to 1:3 every 7 to 10 days
Medium renewal: Every 3 to 4 days
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002
Recommended serum: ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Phosphate-buffered saline: Hs 505.T細(xì)胞ATCC 30-2200
Cell culture tested DMSO: ATCC 4-X
