NCI-H520細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

是否是腫瘤細(xì)胞： 1

物種來源： 人

器官來源： 肺

數(shù)量： 大量

ATCC Number： HTB-182?

相關(guān)**： 鱗狀細(xì)胞癌

Designations: NCI-H520 [H520]

Depositors: AF Gazdar, JD Minna

NCI-H520細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: squamous cell carcinoma

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Applications: transfection host (Roche Transfection Reagents)

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 10,11

D16S539: 8,13

D5S818: 12,13

NCI-H520細(xì)胞D7S820: 8,12

THO1: 10

TPOX: 8

vWA: 18,19

Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 58 occurring at 30%. The frequency of higher ploidies was 3.2%. Over 30 marker chromosomes were common to all cells, and four others were found in some cells. Among the common markers were 1q+, t(1q8q), 2q+, der(16)t(3;16)(q21;q22), der(19)t(13;19)(q21;q13), and der(5)t(5;5)(p15pq13). Generally, there were two copies of der(5) in each cell. Normal Y and D group chromosomes were absent, and the X chromosome was single.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1

PGM3, 1

Gender: male

Comments: The NCI-H520 cell line was derived by A.F. Gazdar and associates in 1982 from a sample of a lung mass taken from a patient with squamous cell carcimoma of the lung.

The cells express greatly reduced levels of p53 mRNA relative to normal lung tissue, but exhibit no gross structural DNA abnormalities.

The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.

The line can be cloned in soft agar (with or without serum).

Propagation: NCI-H520細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Doubling Time: 61 hrs in medium with serum; 25 to 32 hrs in serum-free medium

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1605: Banks-Schlegel SP, et al. Intermediate filament and cross-linked envelope expression in human lung tumor cell lines. Cancer Res. 45: 1187-1197, 1985. PubMed: 2578876

1806: Takahashi T, et al. p53: A frequent target for genetic abnormalities in lung cancer. Science 246: 491-494, 1989. PubMed: 2554494

22434: Brower M, et al. NCI-H520細(xì)胞Growth of cell lines and clinical specimens of human non-small cell lung cancer in a serum-free defined medium. Cancer Res. 46: 798-806, 1986. PubMed: 3940644