RPMI-7951細(xì)胞

細(xì)胞形態(tài)： 上皮樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

ATCC Number： HTB-66?

相關(guān)**： 惡性黑色素瘤

年限： 18 years

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

器官來(lái)源： 皮膚

運(yùn)輸方式： 凍存運(yùn)輸

Designations: RPMI-7951

Depositors: G Moore

RPMI-7951細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: skin

Disease: malignant melanoma

Derived from metastatic site: lymph node

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Isolation: Isolation date: 1971

Tumorigenic: Yes

Antigen Expression: Blood Type A; Rh+

DNA Profile (STR): RPMI-7951細(xì)胞Amelogenin: X

CSF1PO: 12

D13S317: 11,12

D16S539: 11,12

D5S818: 11

D7S820: 11,12

THO1: 9,9.3

TPOX: 8

vWA: 17,19

Cytogenetic Analysis: modal number= 49; range = 47 to 66.

This is a hyperdiploid human cell line with the modal chromosome number of 49, occurring in 24% of cells. Polyploid cells occurred at 22%, which is high. Seven marker chromosomes were common to most cells, including t(10p14q); t(15q17p), t(Xp8p), del(17) (p13.1) and three others. Five others occurred only in some cells. Normal X chromosomes were absent. N14, N17 and N22 were mostly single-copied and N2 had three copies. Fluorescence examination did not show the presence of any Y-like chromosome.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1-2

PGM3, 1

Age: 18 years

Gender: female

Ethnicity: RPMI-7951細(xì)胞Caucasian

Comments: A contaminant identified as Mycoplasma fermentans was eliminated in 1975.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Two to three times weekly

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22536: RPMI-7951細(xì)胞Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22540: . . J. Natl. Cancer Inst. 59: 301-307, 1977.

25093: Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479