P3HR-1細(xì)胞

數(shù)量： 大量

細(xì)胞類型： B**細(xì)胞

年限： 7 years

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HTB-62?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： **瘤

細(xì)胞形態(tài)： **樣

組織來(lái)源： ascites

Designations: P3HR-1

P3HR-1細(xì)胞Depositors: G Trempe, LJ Old

Biosafety Level: 2 [Cells Contain Herpesvirus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Tissue: ascites

Disease: Burkitt's lymphoma

Cell Type: B lymphocyte;

Permits/Forms: P3HR-1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,11

D13S317: 12

D16S539: 10,11

D5S818: 12

D7S820: 8,10

THO1: 7,9

TPOX: 6,8

vWA: 15,19

Isoenzymes: ES-D, 1

G6PD, B

GLO-I, 1

Me-2, 2

PGM1, 1

Age: 7 years

Gender: male

Ethnicity: Black, African

Comments: P3HR-1細(xì)胞The P3HR-1 line is a clonally derived subline of Jiyoye (ATCC CCL-87) which yielded up to 50-85% immunofluorescent positive cells when stained with selected human sera.

Herpes type virus particles (Epstein-Barr virus) were observed in as many as 78% of the cells examined with the electron microscope and a good correlation between presence of viral particles and immunofluorescence was obtained.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; heat-inactivated fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 4 X 10 exp5 cells/ml and maintain at between 4 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: derivative:ATCC CCL-87

References: 1093: Benjamin D, et al. Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

22549: Magrath IT, et al. Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr virus nuclear antigen. II. Surface markers. J. Natl. Cancer Inst. 64: 477-483, 1980. PubMed: 6243722

22760: . . Sci. Rep. Res. Inst. Tohoku Univ. Ser. C Med. 16: 97-101, 1969.

22816: Giraldo G, et al. P3HR-1細(xì)胞Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 4329447

22878: Hinuma Y, et al. Immunofluorescence and herpes-type virus particles in the P3HR-1 Burkitt lymphoma cell line. J. Virol. 1: 1045-1051, 1967. PubMed: 4912237

23216: Giraldo G, et al. Kaposi's sarcoma: a new model in the search for viruses associated with human malignancies. J. Natl. Cancer Inst. 49: 1495-1507, 1972. PubMed: 4346014