SCC-15細(xì)胞

器官來源： 舌頭

ATCC Number： CRL-1623?

相關(guān)**： 鱗狀細(xì)胞癌

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 55 years

數(shù)量： 大量

Designations: SCC-15

Depositors: JG Rheinwald

SCC-15細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: tongue

Disease: squamous cell carcinoma

Cellular Products: epidermal keratins; 40 kD keratin; low levels of involucrin

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,13

D13S317: 9,14

D16S539: 12,15

SCC-15細(xì)胞D5S818: 12

D7S820: 10,11

THO1: 9,9.3

TPOX: 8

vWA: 15,17

Age: 55 years

Gender: male

Comments: Unlike SCC-4 (ATCC CRL-1624), this line does not grow well in semi-solid medium, and does not require a feeder layer.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium containing 1.2 g/L sodium bicarbonate, 2.5 mM L-glutamine, 15 mM HEPES and 0.5 mM sodium pyruvate supplemented with 400 ng/ml hydrocortisone, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: SCC-15細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 23039: Rheinwald JG, Beckett MA. Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultures from human squamous cell carcinomas. Cancer Res. 41: 1657-1663, 1981. PubMed: 7214336

26113: Rheinwald JG, Beckett MA. Defective terminal differentiation in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22: 629-632, 1980. PubMed: 6160916

26114: Wu YJ, Rheinwald JG. A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas. Cell 25: 627-635, 1981. SCC-15細(xì)胞PubMed: 6169443