HuT 78細胞

數(shù)量： 大量

生長狀態(tài)： 懸浮生長

年限： 53 years *****

運輸方式： 凍存運輸

細胞形態(tài)： **樣

ATCC Number： TIB-161?

相關(guān)**： 其他**

細胞類型： 其他細胞類型

是否是腫瘤細胞： 1

物種來源： 人

Designations: HuT 78

HuT 78細胞Depositors: AF Gazdar

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: Sezary Syndrome

Cell Type: cutaneous T lymphocyte;

HuT 78細胞Cellular Products: interleukin 2 [1140]

tumor necrosis factor alpha (TNF alpha) [23420]

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: interleukin 2 (IL-2), expressed [1140]

Tumorigenic: Yes

Antigen Expression: CD4; Homo sapiens [22610]

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 8,12

D16S539: 11,12

D5S818: 11,12

D7S820: 8,11

THO1: 8,9

TPOX: 8,9

vWA: 14,15

Age: HuT 78細胞53 years *****

Gender: male

Ethnicity: Caucasian

Comments: H9 (ATCC HTB-176) is a clonal derivative of HuT 78 [Pubmed: 2567177].

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp4 and 8 X 10 exp5 viable cells/ml. maintain cell density at less than 1 X 10 exp6 cells/ml.

Medium Renewal: Two to three times weekly

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: about 65 hours

Related Products: HuT 78細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

derivative:ATCC HTB-176

References: 1140: Gootenberg JE, et al. Human cutaneous T cell lymphoma and leukemia cell lines produce and respond to T cell growth factor. J. Exp. Med. 154: 1403-1418, 1981. PubMed: 6975346

22484: Mann DL, et al. Origin of the HIV-susceptible human CD4+ cell line H9. AIDS Res. Hum. Retroviruses 5: 253-255, 1989. PubMed: 2567177

22610: Gazdar AF, et al. Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas. Blood 55: 409-417, 1980. PubMed: 6244013

23228: Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674

23420: O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285

32283: Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991

32396: Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128

32796: Bloom TJ, Beavo JA. HuT 78細胞Identification and tissue-specific expression of PDE7 phosphodiesterase splice variants. Proc. Natl. Acad. Sci. USA 93: 14188-14192, 1996. PubMed: 8943082