Y79細(xì)胞
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
器官來(lái)源： 眼
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： HTB-18?
數(shù)量： 大量
相關(guān)**： 其他**
細(xì)胞形態(tài)： 其他
運(yùn)輸方式： 凍存運(yùn)輸
組織來(lái)源： retina
年限： 2.5 years
Designations: Y79
Depositors: B Gallie
Y79細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: multicellular clusters
Source: Organ: eye
Tissue: retina
Disease: retinoblastoma
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Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
Y79細(xì)胞D7S820: 8,9
THO1: 6,9.3
TPOX: 8
vWA: 15,18
Cytogenetic Analysis: hypertriploidy, with abnormalities including dicentrics, breaks, pulverizations and minutes
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
Age: 2.5 years
Gender: female
Ethnicity: Caucasian
Comments: Y79細(xì)胞The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation.
The donor had a strong maternal family history of retinoblastoma.
Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Allow aggregates to settle to the bottom of the flask. Remove supernatant and discard. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22531: Y79細(xì)胞Reid TW, et al. Characteristics of an established cell line of retinoblastoma. J. Natl. Cancer Inst. 53: 347-360, 1974. PubMed: 4135597
32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024
32684: Wong HK, Ziff EB. The human papillomavirus type 16 E7 protein complements adenovirus type 5 E1A amino-terminus-dependent trasactivation of adenovirus type 5 early genes and increases ATF and Oct-1 DNA binding activity. J. Virol. 70: 332-340, 1996. PubMed: 8523545
