A375.S2細胞

器官來源： 皮膚

生長狀態(tài)： 貼壁生長

年限： 54 years

運輸方式： 凍存運輸

數(shù)量： 大量

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： CRL-1872?

相關(guān)**： 惡性黑色素瘤

Designations: A375.S2

A375.S2細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology:

Source: Organ: skin

Disease: malignant melanoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 11,14

D16S539: 9

D5S818: 12

D7S820: 9

THO1: 8

A375.S2細胞TPOX: 8,10

vWA: 16,17

Age: 54 years

Gender: female

Comments: This line was cloned by limiting dilution from the A375 melanoma cell line (see ATCC CRL-1619).

These cells will eventually die and lyse when cultured in medium containing interleukin-1 (interleukin 1, IL-1) and therefore can be used in a reliable and sensitive growth inhibition assay for IL-1.

Sensitivity to IL-1 is maximal when the cells are derived from subconfluent cultures in logarithmic growth.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. A375.S2細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, rinse with fresh 0.25% trypsin, 0.03% EDTA solution and let the culture sit at room temperature (or 37C) until cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-1619

References: 1773: A375.S2細胞Onozaki K, et al. Human interleukin-1 is a cytocidal factor for several tumor cell lines. J. Immunol. 135: 3962-3968, 1985. PubMed: 2415593

1774: Lachman LB, et al. Natural and recombinant human interleukin-1beta is cytotoxic for human melanoma cells. J. Immunol. 136: 3098-3102, 1986. PubMed: 3485680

1775: Nakai S, et al. A simple, sensitive bioassay for the detection of interleukin-1 using human melanoma A375 cell line. Biochem. Biophys. Res. Commun. 154: 1189-1196, 1988. PubMed: 3261584

23218: Giard DJ, et al. In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758