NCI-H446細胞

年限： 61 years

生長狀態(tài)： 混合型生長

運輸方式： 凍存運輸

細胞形態(tài)： 上皮樣

ATCC Number： HTB-171?

相關(guān)**： 小細胞肺癌

是否是腫瘤細胞： 1

物種來源： 人

數(shù)量： 大量

器官來源： 肺

Designations: NCI-H446 [H446]

Depositors: AF Gazdar, JD Minna

NCI-H446細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: lung

Disease: carcinoma; small cell lung cancer

Derived from metastatic site: pleural effusion

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Isolation: Isolation date: 1982

Tumorigenic: Yes

DNA Profile (STR):NCI-H446細胞 Amelogenin: X,Y

CSF1PO: 13,14

D13S317: 8

D16S539: 12

D5S818: 11

D7S820: 10,11

THO1: 8,9.3

TPOX: 9,11

vWA: 18,19

Cytogenetic Analysis: This is a hypertriploid human cell line. The modal chromosome number is 74, occurring at 22% with polyploidy at 2.5%. Over 25 markers were common to most cells including inv(1)(p32q21), der(1)t(1:4)(q42;q11), i(5q), t(2p13q) and der(19)t(7;HSR;19) (q11;HSR;p13.3). Structurally normal N1 and N13 were absent and normal N4, N5 and N10 were mostly single copies per cell. There were two X/Y pairs per cell.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 0

PGM1, 1-2

PGM3, 1

Age: 61 years

Gender: NCI-H446細胞male

Ethnicity: Caucasian

Comments: The NCI-H446 cell line was derived by D. Carney, A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with small cell cancer of the lung.

The morphology of the original tumor was not characteristic of SCLC.

The line is a biochemical and morphological variant of SCLC that expresses neuron specific enolase and the brain isoenzyme of creatine kinase.

It does not have detectable levels of L-DOPA decarboxylase, bombesin, vasopressin, oxytocin or gastrin releasing peptide.

C-myc DNA sequences are amplified about 20 fold, and there is a 15 fold increase in c-myc RNA relative to normal cells.

The line was originally propagated in serum free RPMI 1640 medium supplemented with 10 nM hydrocortisone, 0.005 mg/ml insulin, 0.01 mg/ml transferrin, 10 nM 17-beta-estradiol, and 30 nM sodium selenite, 95%.

Fetal bovine serum, 5%.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: NCI-H446細胞Protocol: This is a cell line that grows as both attached and suspended cells. The suspended cells are viable and can be used for subculture.To subculture the attached cells, remove the old medium (recover the suspended cells by centrifugation if desired), rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA and let the culture sit at 37C until the cells detach. Add fresh medium, disperse cells and transfer to a new flask.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:9 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1805: Little CD, et al. Amplification and expression of the c-myc oncogene in human lung cancer cell lines. Nature 306: 194-196, 1983. PubMed: 6646201

23056: Carney DN, et al. Establishment and identification of small cell lung cancer cell lines having classic and variant features. Cancer Res. 45: 2913-2923, 1985. PubMed: 2985257

23057: Gazdar AF, et al. Characterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological, and growth properties. Cancer Res. 45: 2924-2930, 1985. PubMed: 2985258

23162: Verbeeck MA, et al. Expression of the vasopressin and gastrin-releasing peptide genes in small cell lung carcinoma cell lines. Pathobiology 60: 136-142, 1992. PubMed: 1320893