Malme-3M細(xì)胞

細(xì)胞形態(tài)： 混合型

ATCC Number： HTB-64?

相關(guān)**： 惡性黑色素瘤

生長狀態(tài)： 混合型生長

是否是腫瘤細(xì)胞： 1

物種來源： 人

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

年限： 43 years

Designations: Malme-3M

Depositors: J Fogh

Malme-3M細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed adherent-suspension

Organism: Homo sapiens

Morphology: mixed

Source: Disease: malignant melanoma

Derived from metastatic site: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; FAX (212) 717-3439.

Malme-3M細(xì)胞Tumorigenic: Yes

Antigen Expression: HLA A2, Aw30, B13, B40(+/-), DRw7

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12

D13S317: 8,13

D16S539: 9,12

D5S818: 11

D7S820: 9,12

THO1: 8

TPOX: 8,9

vWA: 15,16

Cytogenetic Analysis: modal number = 88; range = 76 to 93

This is a hypotetraploid human cell line with the modal chromosome number of 88 occurring in 17% of cells. Cells having 82 to 87 chromosome counts were also abundant. Six marker chromosomes were common to most cells, including i(1q), i(6p), i(7q), t(4qter--4pter::?::4q11--4qter) and two others. Malme-3M細(xì)胞Except for the paired i(7q), all were single. Two copies of normal X and Y chromosomes were detected in most cells. Normal chromosomes N3 and N17 had five copies in most cells.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1

PGM3, 1

Age: 43 years

Gender: male

Ethnicity: Caucasian

Comments: This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh.

This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. Thus, the two lines provide tumor and normal counterparts for comparative in vitro studies.

Propagation: Malme-3M細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: .

Remove to a centrifuge tube.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

To remove trypsin-EDTA solution, transfer cell suspension to the centrifuge tube with the medium and cells from step #1 and spin at approximately 125 xg for 5 to 10 minutes.

Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Two to three times weekly

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

derived from same individual:ATCC HTB-102

References: 21869: . Human tumor cells in vitro. New York: Plenum Press; 1975.

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212