TT細(xì)胞
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 1
物種來源： 人
器官來源： 甲狀腺
細(xì)胞形態(tài)： 上皮樣
運輸方式： 凍存運輸
年限： 77 years
ATCC Number： CRL-1803?
相關(guān)**： 腫瘤
組織來源： medulla
TT細(xì)胞Designations: TT
Depositors: SS Leong
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: thyroid
Tissue: medulla
Disease: carcinoma
Cellular Products: calcitonin; carcinoembryonic antigen (CEA) [21867]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. TT細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,13
D13S317: 11
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 6,9
TPOX: 8,11
vWA: 16,18
Cytogenetic Analysis: aneuploid with several markers; modal number = 40; range = 32 to 86 [21867]
Age: 77 years
Gender: female
Ethnicity: Caucasian
Comments: TT細(xì)胞The TT cell line was established by S.S. Leong, et al. from a specimen obtained by needle biopsy from a 77 year old female with thyroid medullary carcinoma. [21867]
TT cells continuously produce high levels of calcitonin and CEA. [21867]
Immunoreactive calcitonin was found to be produced in cell culture at levels of 3900 pg/million cells and 7700 pg/million cells 24 and 72 hours respectively after a medium change. [21867]
CEA was found to accumulate to greater than 27 ng/million cells over a 72 hours period. [21867]
Chromosomal analysis of the cell line and tumors induced in nude mice reveal an aneuploid human karyotype with several marker chromosomes. [21867]
The initial characterization studies of the TT cell line were conducted using early passage TT cells cultivated in RPMI 1640 medium supplemented with 15% fetal bovine serum and 1 mM L-glutamine.
It is not known if the neuropeptides reported to be produced by this cell line when it was grown in RPMI 1640 medium are also produced by the cells when they are cultured in Ham's F12K medium.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 83 hrs
Related Products: TT細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 21867: . Advances in thyroid neoplasia, 1981. Rome: Field Educational Italia; 1981.
32264: Behr TM, et al. Improved treatment of medullary thyroid cancer in a nude mouse model by combined radioimmunochemotherapy: doxorubicin potentiates the therapeutic efficacy of radiolabeled antibodies in a radioresistant tumor type. Cancer Res. 57: 5309-5319, 1997. PubMed: 9393755
