RD-ES細胞

數(shù)量： 大量

是否是腫瘤細胞： 1

物種來源： 人

ATCC Number： HTB-166?

相關**： 其他**

細胞形態(tài)： 上皮樣

生長狀態(tài)： 混合型生長

運輸方式： 凍存運輸

年限： 19 years

器官來源： 骨

Designations: RD-ES

RD-ES細胞Depositors: G Marshall, M Kirchen

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed: adherent and clusters in suspension

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: bone

Disease: Ewing's sarcoma

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Antigen Expression: Blood type B; Rh+

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1-2

Me-2, 1-2

RD-ES細胞PGM1, 1-2

PGM3, 1

Age: 19 years

Gender: male

Ethnicity: Caucasian

Comments: The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewings sarcoma of the humerus.

Ultrastructurally the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter.

The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells.

Propagation: RD-ES細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Shake the flask after removing most of the medium. Add fresh medium and transfer to new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:RD-ES細胞ATCC 30-2020