A7細(xì)胞

ATCC Number： CRL-2500?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 黑色素瘤

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

細(xì)胞形態(tài)： 其他

器官來源： 皮膚

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: A7 [M2A7]

A7細(xì)胞Depositors: TP Stossel

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: melanocytic

Source: Organ: skin

Disease: melanoma

Cellular Products: filamin-1 (actin-binding protein ABP or ABP-280)

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Comments: A7細(xì)胞M2 cells are severely deficient in filamin-1 mRNA and protein. Filamin-1, also known as actin-binding protein (ABP, APB-280), cross-links actin filaments in vitro and connects these filaments to plasma membrane glycoproteins. [42092]

The filament-1 gene on the X chromosome is present in these cells.

ABP (filamin-1) was transfected into M2 cells in a mammalian expression vector (LK444) containing full length human ABP recombinant DNA (LK-APB). [42092]

A7 cells express levels of filamin-1 comparable to wild-type filamin-1 containing melanoma cells. [42092]

Filamin-1 is essential for cell migration and has been shown to bind partners in cell signal transduction, cell membrane sorting and cytoskeleton-membrane association.

A7 cells are useful as a control for studying these systems.

Propagation: ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate supplemented with 10 mM HEPES, 0.5 mg/ml G418 and 10% fetal bovine serum

Temperature: 37.0°C

Subculturing: A7細(xì)胞Protocol: Remove medium, and rinse with 0.25% trypsin - 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: culture medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 42092: Cunningham CC, et al. Actin-binding protein requirement for cortical stability and efficient locomotion. Science 255: 325-327, 1992. PubMed: 1549777

43261: Cunningham CC. Actin polymerization and intracellular solvent flow in cell surface blebbing. J. Cell Biol. 129: 1589-1599, 1995. PubMed: 7790356

43263: Cantiello HF, et al. Actin-binding protein contributes to cell volume regulatory ion channel activation in melanoma cells. J. Biol. Chem. 268: 4596-4599, 1993. PubMed: 8383118

47049: Byers HR, et al. A7細(xì)胞Cell migration and actin organization in cultured human primary, recurrent cutaneous and metastatic melanoma. Time-lapse and image analysis. Am. J. Pathol. 139: 423-435, 1991. PubMed: 1867326