Hs 913T細(xì)胞

生長狀態(tài)： 貼壁生長

ATCC Number： HTB-152?

相關(guān)**： 纖維肉瘤

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 成纖維樣

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 51 years

Designations: Hs 913T

Depositors: RB Owens

Hs 913T細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Disease: fibrosarcoma

Derived from metastatic site: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 12

D13S317: 11

D16S539: 13

D5S818: 10

Hs 913T細(xì)胞D7S820: 10

THO1: 8,9.3

TPOX: 9,11

vWA: 17,18

Cytogenetic Analysis: modal number = 111 to 113; range = 95 to 120; large submetacentric and double minute (DM) markers

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 2

PGM3, 2

Hs 913T細(xì)胞Age: 51 years

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes).

Add fresh medium, aspirate and dispense into new flasks.

Subculture every 6 to 8 days.

Preservation: Hs 913T細(xì)胞Culture medium, 95%; DMSO, 5%