NAMALWA細(xì)胞

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgM; lambda light chain

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： CRL-1432?

相關(guān)**： **瘤

年限： 3 years

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

NAMALWA細(xì)胞Designations: NAMALWA

Depositors: NB Finter

Isotype: IgM; lambda light chain

Biosafety Level: 2 [Cells Contain Herpesvirus ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: Burkitt's lymphoma

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. NAMALWA細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 9

D5S818: 12,13

D7S820: 11

THO1: 7,9.3

TPOX: 6,11

vWA: 14

Cytogenetic Analysis: This is a human cell line with the hypodiploid chromosome count. The modal chromosome number was 44, occurring in 30% of cells. NAMALWA細(xì)胞The rate of cells with higher ploidies was 2.8%. Twelve to 14 marker chromosomes were common to most cells. Among these were ins(1;?) (q11;?), ins(3) (q11q29), del(3) (p12/13), der(6)t(3;6) (p21;p25), t(15q21q) and seven to nine others. Normal N3 was absent, and N7 had three copies per cell. The X chromosome was single. There was no Y chromosome.

Age: 3 years

Gender: female

Comments: The cells contain the Epstein-Barr virus (EBV) genome.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 92.5%; fetal bovine serum, 7.5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Cultures should be started at 5 X 10(5) viable cells/ml and subcultured at 2 X 10(6) cells/ml.

Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1059: Klein F, et al. Large-scale production and concentration of human lymphoid interferon. Antimicrob. Agents Chemother. 15: 420-427, 1979. PubMed: 464569

1093: Benjamin D, et al. NAMALWA細(xì)胞Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type. J. Immunol. 129: 1336-1342, 1982. PubMed: 6286763

22364: Klein G, et al. Sensitivity of Epstein-Barr virus (EBV) producer and non-producer human lymphoblastoid cell lines to superinfection with EB-virus. Int. J. Cancer 10: 44-57, 1972. PubMed: 4122458

58035: Nyormoi O, et al. Sensitivity to EBV superinfection and IUdR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. Int. J. Cancer 12: 396-408, 1973. PubMed: 4365093