MSTO-211H細胞
生長狀態(tài)： 貼壁生長
年限： 62 years
是否是腫瘤細胞： 1
物種來源： 人
ATCC Number： CRL-2081?
相關(guān)**： 其他**
運輸方式： 凍存運輸
細胞形態(tài)： 成纖維樣
數(shù)量： 大量
Designations: MSTO-211H
Depositors: G Bepler
Biosafety Level: 1
MSTO-211H細胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Disease: biphasic mesothelioma
Derived from metastatic site: lung
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Isolation: Isolation date: 1985
Tumorigenic: Yes
Oncogene: c-myc +; v-src +; v-abl +; v-erb B +; c-raf 1 +; H-ras +; K-ras +; N-ras +; N-myc -; L-myc -; c-myb -; c-fos -; v-fes -; v-fms -; v-sis -
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
MSTO-211H細胞D16S539: 13
D5S818: 12
D7S820: 8,12
THO1: 8,9.3
TPOX: 11
vWA: 16,18
Cytogenetic Analysis: modal number = 72; range = 70 to 78
Age: 62 years
Gender: male
Ethnicity: Caucasian
Comments: The MSTO-211H cell line was established in 1985 from the pleural effusion of a patient with biphasic mesothelioma of the lung.
The patient had not received prior radiation or chemotherapy.
MSTO-211H cells have high affinity binding sites for EGF, and express Neuron specific enolase (NSE) and both the alpha and beta subunits of human chorionic gonadotropin (HCG).
L-DOPA decarboxylase (DDC), bombesin and neurotensin were not detected.
The cells overexpress the c-myc protooncogene, and no gene rearrangement or amplification was observed.
They are positive for the expression of v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras.
MSTO-211H細胞Expression of N-myc, L-myc, c-myb, c-fos, v-fes, v-fms and v-sis oncogene expression was not detected.
The cells can reach a saturation density of 400000 cells per sq cm, but will slough off of the surface as they attain this density.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 20 hrs
Related Products: Recommended medium MSTO-211H細胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22261: Bepler G, Koehler A. Multiple chromosomal aberrations and 11p allelotyping in lung cancer cell lines. Cancer Genet. Cytogenet. 84: 39-45, 1995. PubMed: 7497441
22745: Bepler G, et al. Characterization of the state of differentiation of six newly established human non-small-cell lung cancer cell lines. Differentiation 37: 158-171, 1988. PubMed: 2840315
23067: Haeder M, et al. Epidermal growth factor receptor expression in human lung cancer cell lines. Cancer Res. 48: 1132-1136, 1988. PubMed: 2830015
24389: . . Lung Cancer 4: 155-161, 1988.
