MES-SA/Dx5細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
細(xì)胞形態(tài)： 成纖維樣
年限： 56 years
器官來(lái)源： **
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
ATCC Number： CRL-1977?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
Designations: MES-SA/Dx5
MES-SA/Dx5細(xì)胞Depositors: BI Sikic
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: uterus
Disease: uterine sarcoma
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Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 11
D13S317: 13
D16S539: 11,12
MES-SA/Dx5細(xì)胞D5S818: 13
D7S820: 7,11
THO1: 6
TPOX: 8,11
vWA: 18
Cytogenetic Analysis: modal number = 47; the karyotype is 47XX, 12q+, 22q-, t(5q,6p), -3, -4, -7, +t(3q?,7q), Mar 1, + Mar 2, + Mar 3; the two additional marker chromosomes indicate clonal selection during drug selection
Age: 56 years
Gender: female
Ethnicity: Caucasian
Comments: The multiple drug resistant cell line MES-SA/Dx5 was established from MES-SA cells (ATCC CRL-1976) which were grown in the presence of increasing concentrations of doxorubicin.
The cells exhibit a marked cross resistance to a number of chemotherapeutic agents including vinblastine, taxol, colchicine, vincristine, etoposide, dactinomycin, mitoxantrone and daunorubicin.
They exhibit moderate cross resistance to mitomycin C and melphalan.
Resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.
The cells express high levels of mdr-1 mRNA and P-glycoprotein.
Propagation: MES-SA/Dx5細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: Newborn calf serum may be substituted for fetal bovine serum.
Subculturing: Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water).
Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand.
Add fresh medium, aspirate and dispense into new flasks.
Doubling Time: 30 hrs
Related Products: parental cell line:ATCC CRL-1976
References: 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344
23058: Harker WG, Sikic BI. MES-SA/Dx5細(xì)胞Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. PubMed: 4028002
