MES-SA/Dx5細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

細(xì)胞形態(tài)： 成纖維樣

年限： 56 years

器官來(lái)源： **

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： CRL-1977?

相關(guān)**： 其他**

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Designations: MES-SA/Dx5

MES-SA/Dx5細(xì)胞Depositors: BI Sikic

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: uterus

Disease: uterine sarcoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 11

D13S317: 13

D16S539: 11,12

MES-SA/Dx5細(xì)胞D5S818: 13

D7S820: 7,11

THO1: 6

TPOX: 8,11

vWA: 18

Cytogenetic Analysis: modal number = 47; the karyotype is 47XX, 12q+, 22q-, t(5q,6p), -3, -4, -7, +t(3q?,7q), Mar 1, + Mar 2, + Mar 3; the two additional marker chromosomes indicate clonal selection during drug selection

Age: 56 years

Gender: female

Ethnicity: Caucasian

Comments: The multiple drug resistant cell line MES-SA/Dx5 was established from MES-SA cells (ATCC CRL-1976) which were grown in the presence of increasing concentrations of doxorubicin.

The cells exhibit a marked cross resistance to a number of chemotherapeutic agents including vinblastine, taxol, colchicine, vincristine, etoposide, dactinomycin, mitoxantrone and daunorubicin.

They exhibit moderate cross resistance to mitomycin C and melphalan.

Resistance to bleomycin, cisplatin, carmustine, 5-fluorouracil or methotrexate was not observed.

The cells express high levels of mdr-1 mRNA and P-glycoprotein.

Propagation: MES-SA/Dx5細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Growth Conditions: Newborn calf serum may be substituted for fetal bovine serum.

Subculturing: Protocol: Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 ml double distilled water).

Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand.

Add fresh medium, aspirate and dispense into new flasks.

Doubling Time: 30 hrs

Related Products: parental cell line:ATCC CRL-1976

References: 23048: Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344

23058: Harker WG, Sikic BI. MES-SA/Dx5細(xì)胞Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. PubMed: 4028002