SNU-423細胞
是否是腫瘤細胞： 1
物種來源： 人
運輸方式： 凍存運輸
ATCC Number： CRL-2238?
相關(guān)**： 其他**
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
器官來源： 肝
年限： grade III/IV
數(shù)量： 大量
Designations: SNU-423
Depositors: J Park
SNU-423細胞Biosafety Level: 2 [CELLS CONTAIN HEPATITIS B VIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: liver
Tumor Stage: grade III/IV
Disease: pleomorphic hepatocellular carcinoma
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Antigen Expression: Blood Type B; Rh +
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 10,13
D16S539: 9
D5S818: 10
D7S820: 12
SNU-423細胞THO1: 6,9
TPOX: 8
vWA: 15
Cytogenetic Analysis: aneuploid; modal number = 79
Age: 40 years
Gender: male
Ethnicity: Asian
Comments: SNU-423 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient who had been treated by transcatheter arterial embolization with lipoidol plus doxorubicin.
Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat-inactivated fetal bovine serum.
After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Grossly, the original tumor was single nodular with perinodular extensions.
Histologically, it was trabecular type.
SNU-423細胞The cultured cells are multinucleated.
Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization.
HBV genomic RNA was not expressed.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 72 hrs
References: 22872: Park JG, et al. SNU-423細胞Characterization of cell lines established from human hepatocellular carcinoma. Int. J. Cancer 62: 276-282, 1995. PubMed: 7543080
