SW 684細胞

年限： 68 years

運輸方式： 凍存運輸

生長狀態(tài)： 貼壁生長

是否是腫瘤細胞： 1

物種來源： 人

組織來源： connective tissue

數(shù)量： 大量

ATCC Number： HTB-91?

相關(guān)**： 纖維肉瘤

細胞形態(tài)： 成纖維樣

Designations: SW 684 [SW-684, SW684]

Depositors: A Leibovitz

SW 684細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Tissue: connective tissue

Disease: fibrosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Temple Texas, United States

Isolation date: 1974

Tumorigenic: Yes

Cytogenetic Analysis: SW 684細胞hypertriploid; modal number = 73; range = 59 to 79. The rate of higher ploidies was 9.1%. Eleven markers were common to most cells. These include: der(2)t(2;6)(p13;q13), der(12)t(8;12)(q11;q24), t(15q21q), 19q+, t(8p21q?) and six others. Of these, the der(2) and t(8p21q?) were generally paired. A few cells had double minutes (DM) (one per cell when present). There were 4 copies of N1, N18, N20 and N22 in most cells. Normal 15 and Y were absent. The X was paired in all cells.

Isoenzymes: AK-1, 1-2

G6PD, B

GLO-I, 2

PGM1, 1-2

PGM3, 1

Age: 68 years

Gender: male

Ethnicity: Caucasian

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol:

SW 684細胞Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.35 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. SW 684細胞One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080