SK-N-AS細(xì)胞

ATCC Number： CRL-2137?

器官來源： 大腦

相關(guān)**： 神經(jīng)母細(xì)胞瘤

細(xì)胞形態(tài)： 上皮樣

細(xì)胞類型： 其他細(xì)胞類型

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來源： 人

數(shù)量： 大量

年限： 6 years

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: SK-N-AS

SK-N-AS細(xì)胞Depositors: C Helson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: brain

Disease: neuroblastoma

Derived from metastatic site: bone marrow

Cell Type: neuroblast;

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Isolation: Isolation date: 1981

Receptors: insulin-like growth factor I (IGF-I), expressed

Tumorigenic: SK-N-AS細(xì)胞Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 9

D16S539: 14

D5S818: 11,12

D7S820: 11,13

THO1: 9.3

TPOX: 11,12

vWA: 16,17

Age: 6 years

Gender: female

Ethnicity: Caucasian

Comments: SK-N-AS cells synthesize Insulin-like growth factor II (IFG-II) and IGF-II RNA and possess type I IGF receptors [Pubmed ID: 2547840]. Retinoic acid partially inhibits proliferation, and cells fail to differentiate [Pubmed ID: 1751405]. The cells exhibit low or no MDR1 expression.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:

SK-N-AS細(xì)胞O.1 mM Non-Essential Amino Acids (NEAA)

fetal bovine serum to a final concentration of 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 MM EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 39 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 22290: Sugimoto T, et al. Determination of cell surface membrane antigens common to both human neuroblastoma and leukemia-lymphoma cell lines by a panel of 38 monoclonal antibodies. J. Natl. Cancer Inst. 73: 51-57, 1984. PubMed: 6610792

22439: Iavarone A, et al. Uptake and storage of m-iodobenzylguanidine are frequent neuronal functions of human neuroblastoma cell lines. Cancer Res. 53: 304-309, 1993. PubMed: 8417824

22444: Gaetano C, et al. Retinoic acid negatively regulates p34cdc2 expression during human neuroblastoma differentiation. Cell Growth Differ. 2: 487-493, 1991. PubMed: 1751405

22500: El-Badry OM, et al. SK-N-AS細(xì)胞Autonomous growth of a human neuroblastoma cell line is mediated by insulin-like growth factor II. J. Clin. Invest. 84: 829-839, 1989. PubMed: 2547840