Detroit 562細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 上皮樣

年限： *****

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

ATCC Number： CCL-138?

相關(guān)**： 腫瘤

器官來源： 咽

是否是腫瘤細(xì)胞： 1

物種來源： 人

Designations: Detroit 562

Detroit 562細(xì)胞Depositors: CS Stulberg

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: pharynx

Disease: carcinoma

Derived from metastatic site: pleural effusion

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Virus Susceptibility: Detroit 562細(xì)胞Human poliovirus 1

Vesicular stomatitis virus

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 12

D16S539: 11

D5S818: 11,12

D7S820: 8,10

THO1: 8,9

TPOX: 8,10

vWA: 16

Cytogenetic Analysis: Modal number = 64; range = 58 to128

A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell.

Isoenzymes: G6PD, B

Age: *****

Gender: female

Ethnicity: Detroit 562細(xì)胞Caucasian

Comments: The cells are positive for keratin by immunoperoxidase staining.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Detroit 562細(xì)胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 1071: Peterson WD Jr., et al. Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms. Proc. Soc. Exp. Biol. Med. 128: 772-776, 1968. PubMed: 5668122

1072: Peterson WD Jr., et al. A permanent heteroploid human cell line with type B glucose-6-phosphate dehydrogenase. Proc. Soc. Exp. Biol. Med. 136: 1187-1191, 1971. PubMed: 5554463