BC-3細(xì)胞

生長狀態(tài)： 懸浮生長

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2277?

相關(guān)**： **瘤

年限： 85 years

數(shù)量： 大量

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

細(xì)胞形態(tài)： **樣

Designations: BC-3

BC-3細(xì)胞Depositors: Cornell Res. Fndn., Inc.

Biosafety Level: 2 [Cells contain Kaposi's sarcoma-associated herpesvirus genome ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: lymphoma

Cell Type: B lymphocyte;

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Applications: transfection host (Roche Transfection Reagents)

Antigen Expression: CD30 +; CD38 +; CD45 +; CD 54 +; CD71 +; HLA-DR +; EMA + (epithelial membrane antigen); CD2 -; CD3 -; CD4 -; CD5 -, CD8 -; CD19 -; CD20 -; CD21 -; CD22 -

DNA Profile (STR): BC-3細(xì)胞Amelogenin: X

CSF1PO: 11,12

D13S317: 11

D16S539: 12

D5S818: 11,12

D7S820: 10,12

THO1: 6,9

TPOX: 8,11

vWA: 14,18

Age: 85 years

Gender: male

Ethnicity: Caucasian

Comments: BC-3 is a primary effusion lymphoma cell line originated in 1995 by Leandros Arvanitakis and Ethel Cesarman.

The cells were established from pleural effusion from a human immunodeficiency virus (HIV) negative patient diagnosed with primary effusion (body cavity based) lymphoma (PEL).

The BC-3 cell line contains the viral genome for Kaposi's sarcoma-associated herpesvirus (KSHV, provisionally designated HHV-8).

BC-3細(xì)胞KSHV is a recently identified and partially uncharacterized virus.

BC-3 cells are negative for both HIV and Epstein-Barr virus (EBV).

They are also reported to be negative for herpes simplex virus 1 & 2 (HSV-1 and HSV-2) and cytomegalovirus (CMV).

The BC-3 cell line allows in vitro culture of KSHV viral genomes in the absence of EBV.

It is ideal for the isolation and study of KSHV.

BC-3 has an intact KSHV genome of about 170 kb both in the cell line and in the viral isolate.

The cells do not express B-cell lineage restricted antigens or kappa or lambda immunoglobulin light chains) or T-cell lineage-restricted antigens.

The cells do express activation antigens.

Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23359: Arvanitakis L, et al. BC-3細(xì)胞Establishment and characterization of a primary effusion (body cavity- based) lymphoma cell line (BC-3) harboring kaposi's sarcoma-associated herpesvirus (KSHV/HHV-8) in the absence of Epstein-Barr virus. Blood 88: 2648-2654, 1996. PubMed: 8839859

39036: Cesarman E, et al. KSHV positive cell lines. US Patent 5,908,773 dated Jun 1 1999