DBTRG-05MG細(xì)胞

器官來(lái)源： 大腦

細(xì)胞形態(tài)： 成纖維樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 59 years

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞類型： 其他細(xì)胞類型

ATCC Number： CRL-2020?

相關(guān)**： 其他**

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

Designations: DBTRG-05MG

Depositors: CA Kruse

DBTRG-05MG細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: brain

Disease: glioblastoma

Cell Type: glial cell;

Cellular Products: vimentin; S-100 protein; neuron specific enolase

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Receptors: epidermal growth factor (EGF)

Antigen Expression: Class I antigen expressed

DNA Profile (STR): DBTRG-05MG細(xì)胞Amelogenin: X

CSF1PO: 10,11

D13S317: 9

D16S539: 10,12

D5S818: 12,13

D7S820: 11

THO1: 7,8

TPOX: 8

vWA: 15,16

Cytogenetic Analysis: near tetraploid; range 87 to 91; most cells were missing copies of chromosome 10 and had extra copies of chromosome 7

Age: 59 years

Gender: female

Ethnicity: Caucasian

Comments: The DBTRG-O5MG (Denver Brain Tumor Research Group 05) cell line was established from tissue from a patient with glioblastoma multiforme who had been treated with local brain irradiation and multidrug chemotherapy.

The cells are negative for platelet derived growth factor (PDGF), neuronal cell adhesion molecule (NCAM), glial fibrillary acid protein (GFAP) and class II antigen (HLA DR).

No loss of heterozygosity in the p53 tumor suppressor gene was detected.

Propagation: DBTRG-05MG細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

additional 30 mg/L L-proline

additional 35 mg/L L-cystine

additional 3.57 g/L HEPES

15 mg/L hypoxanthine

1 mg/L adenosine triphosphate

10 mg/L adenine

1 mg/L thymidine

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, add fresh 0.25% trypsin, rinse and remove trypsin. Let the flask sit at room temperature (or incubate at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

References: 24397: Kruse CA, et al. Characterization of a continuous human glioma cell line DBTRG-05MG: growth kinetics, karyotype, receptor expression, and tumor suppressor gene analyses. In Vitro Cell. Dev. Biol. 28A: 609-614, 1992. PubMed: 1331021

32274: Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765

32550: Debinski W, et al. DBTRG-05MG細(xì)胞Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406