DBTRG-05MG細(xì)胞
器官來(lái)源： 大腦
細(xì)胞形態(tài)： 成纖維樣
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 59 years
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞類型： 其他細(xì)胞類型
ATCC Number： CRL-2020?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 1
物種來(lái)源： 人
Designations: DBTRG-05MG
Depositors: CA Kruse
DBTRG-05MG細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: brain
Disease: glioblastoma
Cell Type: glial cell;
Cellular Products: vimentin; S-100 protein; neuron specific enolase
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Receptors: epidermal growth factor (EGF)
Antigen Expression: Class I antigen expressed
DNA Profile (STR): DBTRG-05MG細(xì)胞Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 10,12
D5S818: 12,13
D7S820: 11
THO1: 7,8
TPOX: 8
vWA: 15,16
Cytogenetic Analysis: near tetraploid; range 87 to 91; most cells were missing copies of chromosome 10 and had extra copies of chromosome 7
Age: 59 years
Gender: female
Ethnicity: Caucasian
Comments: The DBTRG-O5MG (Denver Brain Tumor Research Group 05) cell line was established from tissue from a patient with glioblastoma multiforme who had been treated with local brain irradiation and multidrug chemotherapy.
The cells are negative for platelet derived growth factor (PDGF), neuronal cell adhesion molecule (NCAM), glial fibrillary acid protein (GFAP) and class II antigen (HLA DR).
No loss of heterozygosity in the p53 tumor suppressor gene was detected.
Propagation: DBTRG-05MG細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
additional 30 mg/L L-proline
additional 35 mg/L L-cystine
additional 3.57 g/L HEPES
15 mg/L hypoxanthine
1 mg/L adenosine triphosphate
10 mg/L adenine
1 mg/L thymidine
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, rinse and remove trypsin. Let the flask sit at room temperature (or incubate at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
References: 24397: Kruse CA, et al. Characterization of a continuous human glioma cell line DBTRG-05MG: growth kinetics, karyotype, receptor expression, and tumor suppressor gene analyses. In Vitro Cell. Dev. Biol. 28A: 609-614, 1992. PubMed: 1331021
32274: Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
32550: Debinski W, et al. DBTRG-05MG細(xì)胞Receptor for interleukin (IL) 13 does not interact with IL4 but receptor for IL4 interacts with IL13 on human glioma cells. J. Biol. Chem. 271: 22428-22433, 1996. PubMed: 8798406
