MG-63細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

年限： 14 years

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來源： 骨

ATCC Number： CRL-1427?

相關(guān)**： 骨肉瘤

是否是腫瘤細(xì)胞： 1

物種來源： 人

運(yùn)輸方式： 凍存運(yùn)輸

Designations: MG-63

Depositors: A Billiau

MG-63細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Organ: bone

Disease: osteosarcoma

Cellular Products: interferon

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Receptors: transforming growth factor beta (TGF-beta) RI, expressed

transforming growth factor beta (TGF-beta) RII, expressed

DNA Profile (STR): MG-63細(xì)胞Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11

D16S539: 11

D5S818: 11,12

D7S820: 10

THO1: 9.3

TPOX: 8,11

vWA: 16,19

Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number was 66 occurring in 44% of cells. The rate of cells with higher ploidies was 2.0%. Eighteen to 19 marker chromosomes were common to all cells.

Age: 14 years

Gender: male

Ethnicity: Caucasian

Comments: High levels of interferon production can be induced using polyinosinic - polycytidylic acid, cycloheximide and actinomycin D.

Propagation: MG-63細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

References: 1058: Billiau A, et al. MG-63細(xì)胞Human interferon: mass production in a newly established cell line, MG-63. Antimicrob. Agents Chemother. 12: 11-15, 1977. PubMed: 883813

23014: Takeuchi Y, et al. Relationship between actions of transforming growth factor (TGF)-beta and cell surface expression of its receptors in clonal osteoblastic cells. J. Cell. Physiol. 162: 315-321, 1995. PubMed: 7860639

32445: Yee A, et al. Biochemical characterization of the human cyclin-dependent protein kinase activating kinase. J. Biol. Chem. 271: 471-477, 1996. PubMed: 8550604