A-253細胞

數(shù)量： 大量

細胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

ATCC Number： HTB-41?

運輸方式： 凍存運輸

相關(guān)**： 上皮細胞癌

年限： 54 years *****

器官來源： 其他

是否是腫瘤細胞： 1

物種來源： 人

Designations: A-253

A-253細胞Depositors: G Todaro

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: submaxillary salivary gland

Disease: epidermoid carcinoma

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Tumorigenic: Yes

DNA Profile (STR): Amelogenin: X

CSF1PO: 12,13

D13S317: 9,12

D16S539: 12

D5S818: 11

D7S820: 10,12

A-253細胞THO1: 6,8

TPOX: 8,12

vWA: 16

Cytogenetic Analysis: near triploid with at least 6 markers

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1

PGM3, 1

Age: 54 years *****

Gender: male

Ethnicity: Caucasian

Comments: The A253 line is one of thirteen to be isolated and partially characterized through trial studies on a series of 200 human tumors.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: A-253細胞Remove medium, rinse with fresh 0.25% trypsin, 0.53 mM EDTA solution, remove the trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Interval: every 6 to 8 days

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

23093: Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216

23218: Giard DJ, et al. In vitro cultivation of human tumors: A-253細胞establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51: 1417-1423, 1973. PubMed: 4357758