T84細胞
器官來源： 結腸
細胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
數(shù)量： 大量
是否是腫瘤細胞： 1
物種來源： 人
年限： 72 years
運輸方式： 凍存運輸
ATCC Number： CCL-248?
T84細胞相關**： 大腸癌
Designations: T84
Depositors: H Masui
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: colorectal carcinoma
Derived from metastatic site: lung
Cellular Products: T84細胞carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin
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Applications: transfection host
Receptors: neurotransmitter, expressed
peptide hormone, expressed
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 10
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 6,9
TPOX: 8,11
vWA: 17,18
Cytogenetic Analysis: T84細胞The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal X and chromosome 13 were absent; chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied. No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1-2
PGM1, 1
PGM3, 1
Age: 72 years
Gender: male
Comments: T84細胞The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. Tumor tissue was inoculated subcutaneously and serially transplanted in BALB/c nude mice.
The original histological characteristics of the colon carcinoma were maintained throughout transplantation in nude mice.
After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells.
They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport.
This line exhibits tight junctions, and desmosomes between adjacent cells.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: ATCC complete growth medium: A 1:1 mixture of Ham's F12 medium and Dulbecco's modified Eagle's medium with 2.5 mM L-glutamine, 95%; fetal bovine serum, 5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The cells should be maintained at high density (at least 1/4 confluency).
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006
recommended serum:ATCC 30-2020
References: 1155: Dharmsathaphorn K, et al. A human colonic tumor cell line that maintains vectorial electrolyte transport. Am. J. Physiol. 246: G204-G208, 1984. PubMed: 6141741
26072: Murakami H, Masui H. Hormonal control of human colon carcinoma cell growth in serum-free medium. Proc. Natl. Acad. Sci. USA 77: 3464-3468, 1980. PubMed: 6932031
32376: White LJ, et al. Attachment and entry of recombinant norwalk virus capsids to cultured human and animal cell lines. J. Virol. 70: 6589-6597, 1996. PubMed: 8794293
