BxPC-3細(xì)胞
器官來源： 胰腺
年限： 61 years
數(shù)量： 大量
ATCC Number： CRL-1687?
相關(guān)**： 腺癌
運輸方式： 凍存運輸
細(xì)胞形態(tài)： 上皮樣
生長狀態(tài)： 貼壁生長
是否是腫瘤細(xì)胞： 1
物種來源： 人
BxPC-3細(xì)胞Designations: BxPC-3
Depositors: MH Tan
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: pancreas
Disease: adenocarcinoma
Cellular Products: mucin; pancreas cancer specific antigen (pancreas cancer associated antigen); carcinoembryonic antigen (CEA)
Permits/Forms: BxPC-3細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 13
D13S317: 11
D16S539: 9,11
D5S818: 11
D7S820: 10,13
THO1: 9
TPOX: 8
vWA: 14,18
Age: 61 years
BxPC-3細(xì)胞Gender: female
Comments: The cells do not express the cystic fibrosis transmembrane conductance regulator (CFTR).
A CFTR positive pancreatic line, Capan-1 is available (ATCC HTB-79).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22737: BxPC-3細(xì)胞Loor R, et al. Use of pancreas-specific antigen in immunodiagnosis of pancreatic cancer. Clin. Lab. Med. 2: 567-578, 1982. PubMed: 6754226
23079: Lan MS, et al. Polypeptide core of a human pancreatic tumor mucin antigen. Cancer Res. 50: 2997-3001, 1990. PubMed: 2334903
23153: Chambers JA, Harris A. Expression of the cystic fibrosis gene and the major pancreatic mucin gene, MUC1, in human ductal epithelial cells. J. Cell Sci. 105: 417-422, 1993. PubMed: 7691840
58045: Tan MH, et al. Characterization of a new primary human pancreatic tumor line. Cancer Invest. 4: 15-23, 1986. PubMed: 3754176
