LS123細(xì)胞

是否是腫瘤細(xì)胞： 1

物種來源： 人

器官來源： 結(jié)腸

生長狀態(tài)： 貼壁生長

運(yùn)輸方式： 凍存運(yùn)輸

年限： Dukes' type B

ATCC Number： CCL-255?

相關(guān)**： 大腸癌

數(shù)量： 大量

細(xì)胞形態(tài)： 上皮樣

Designations: LS123

Depositors: LP Rutzky

LS123細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Tumor Stage: Dukes' type B

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA), 9 ng/ml per 10 exp6 cells per 10 days; mucin

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Isolation: Isolation date: March, 1974

Tumorigenic: No

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,11

D13S317: 9,13

D16S539: 13

D5S818: 11

D7S820: 9

THO1: 7,8

TPOX: 9

vWA: 16,17

Cytogenetic Analysis: modal number = 63; range = 54 to 70.

LS123細(xì)胞The cells consistently have 18 to 20 marker chromosomes. They are: (t(1;?), del(1)(p13), del(2) (p11), t(2;?), t(7p, 19p), t(8;?), t(11;11) (p15;q13), t(14q,21q), t(6p,16p), t(X;?), and 10-12 others. There is only one copy per cell of structurally unchanged normal chromosomes for Nos. 1,2, 4, 10, 14, 16, and 18. No. 20 generally has 4 copies per cell and the X has 3 copies per cell. No Y chromosome was detected.

Isoenzymes: AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1-2

Age: 65 years

Gender: female

Ethnicity: Caucasian

Comments: The epithelial cell line, LS123, was established by L.P. Rutzky et al, in 1974 from the second in a series of three primary colonic tumors resected from a 65 year old Caucasian female.

In 1950, a primary colon tumor was resected from the patient (41 years old at that time).

24 years later a second primary tumor classified as a Dukes' type B mucin producing colon adenocarcinoma was excised and the cells were cultured.

LS123細(xì)胞A third primary tumor was discovered in 1980 in an area just proximal to the colostomy and was resected.

The established cell line is aneuploid, releases small amounts of carcinoembryonic antigen, fails to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells.

LS123 cells grow as confluent cell monolayers in either low serum or serum free medium.

In the chick embryonic skin assay, the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness.

Although LS123 exhibits several properties associated with neoplasia, they have little invasive potential and may represent an early stage in tumor development.

The plating efficiency is 12% in the medium recommended below.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: LS123細(xì)胞A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 2157: Rutzky LP, et al. Characterization of a human colonic adenocarcinoma cell line, LS 123. In Vitro 19: 99-107, 1983. PubMed: 6826200