U-138 MG細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 多邊形
生長狀態(tài)： 貼壁生長
ATCC Number： HTB-16?
數(shù)量： 大量
器官來源： 大腦
相關(guān)**： 其他**
年限： : classified as grade IV as of 2007
是否是腫瘤細(xì)胞： 1
物種來源： 人
U-138 MG細(xì)胞Designations: U-138 MG
Depositors: J Ponten
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: polygonal
Source: Organ: brain
Disease: glioblastoma
Tumor Stage: classified as grade IV as of 2007
Permits/Forms: U-138 MG細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Antigen Expression: Blood Type A; Rh+
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18
Cytogenetic Analysis: U-138 MG細(xì)胞Hyperdiploid to pentaploid with several markers; the stemline chromosome number is near triploid with the 2S component occurring at 9.8%., Five markers [t(11;5), t(8q;4), t(19;?18), M1 and M2] were common to most S metaphases. One chromosome 4 could be found in every S metaphase. Chromosome composition was very uniform among cells.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1-2
Me-2, 1
PGM1, 1
PGM3, 1
Age: 47 years
Gender: male
Ethnicity: Caucasian
Comments: This is one of a number of cell lines derived from malignant gliomas (see also ATCC HTB-14 and ATCC HTB-15 ) by J. Ponten and associates from 1966 to 1969.It differs from ATCC HTB-14 in morphology and it has a slower proliferation rate.Mycoplasma contamination was observed and cured by March 1974.NOTE: The two glioblastoma cell lines, U-118 MG (HTB-15) and U-138 MG (HTB-16), reportedly from different individuals have identical VNTR and similar STR patterns.U-118 MG and U-138 MG are very similar cytogenetically and share at least six derivative marker chromosomes.
Propagation: U-138 MG細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22159: Beckman G, et al. G-6-PD and PGM phenotypes of 16 continuous human tumor cell lines. Evidence against cross-contamination and contamination by HeLa cells. Hum. Hered. 21: 238-241, 1971. PubMed: 4332744
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
23094: Olopade OI, et al. U-138 MG細(xì)胞Molecular analysis of deletions of the short arm of chromosome 9 in human gliomas. Cancer Res. 52: 2523-2529, 1992. PubMed: 1568221
23128: Ponten J, Macintyre EH. Long term culture of normal and neoplastic human glia. Acta Pathol. Microbiol. Scand. 74: 465-486, 1968. PubMed: 4313504
32274: Koochekpour S, et al. Met and hepatocyte growth factor/scatter factor expression in human gliomas. Cancer Res. 57: 5391-5398, 1997. PubMed: 9393765
32276: Cairns P, et al. Genomic organization and mutation analysis of Hel-N1 in lung cancers with chromosome 9p21 deletions. Cancer Res. 57: 5356-5359, 1997. PubMed: 9393760
