SW1463細(xì)胞

年限： Dukes' type C

ATCC Number： CCL-234?

相關(guān)**： 大腸癌

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

數(shù)量： 大量

器官來(lái)源： 直腸

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 1

物種來(lái)源： 人

SW1463細(xì)胞Designations: SW1463 [SW 1463, SW-1463]

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: rectum

Tumor Stage: Dukes' type C

Disease: colorectal adenocarcinoma

Cellular Products: carcinoembryonic antigen (CEA) 223 ng/10 exp6 cells/10 days; keratin

Permits/Forms: SW1463細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Antigen Expression: blood type A; Rh +

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 12,13

D16S539: 11

D5S818: 13,14

D7S820: 9

THO1: 6,7

TPOX: 8,11

vWA: 16

Isoenzymes: ES-D, 1

G6PD, B

PEP-D, 1

SW1463細(xì)胞PGD, A

PGM1, 1

PGM3, 1-2

Age: 66 years

Gender: female

Ethnicity: Caucasian

Comments: The line was derived from a grade II mucous producing and grade III solid tumor.

CSAp negative (CSAp-).

The cells are positive for keratin by immunoperoxidase staining.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended

Medium Renewal: 1 to 2 times per week

Remove medium, add fresh 0.25% trypsin, 0.02% EDTA for 10 to 20 minutes at 37C. Add fresh medium, disperse cells and centrifuge to pellet the cells.

Resuspend in fresh medium and dispense into new flasks.

Subculture just prior to confluency (about 10 to 12 days).

References: 23025: Leibovitz A, et al. SW1463細(xì)胞Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36: 4562-4569, 1976. PubMed: 1000501