RT4細(xì)胞

數(shù)量： 大量

器官來源： 膀胱

是否是腫瘤細(xì)胞： 1

物種來源： 人

年限： 63 years

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： HTB-2?

相關(guān)**： 其他**

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

RT4細(xì)胞Designations: RT4

Depositors: C Rigby, LM Franks

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: urinary bladder

Disease: transitional cell papilloma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RT4細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche Transfection Reagents)

Tumorigenic: Yes

Antigen Expression: HLA A25(10), A3, B12, Cw3; Blood Type O

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 8

D16S539: 9

D5S818: 11,12

D7S820: 9,12

THO1: 9,9.3

TPOX: 8,11

vWA: 14,17

Cytogenetic Analysis: RT4細(xì)胞modal number = 49; range = 42 to 55 The cell line is aneuploid human male, with chromosome counts in the near-diploid range. However, the near-tetraploid population may become predominant within very few passages. The 48 and 49 chromosome karyotypes had a single X and a single Y chromosome. Three of the karyotypes at higher ploidy had two X chromosomes and a single Y chromosome. Four marker chromosomes were identified: del(6)(q21), del(10)(p11), 14p+, 12p+. Marker chromosome M4 was not seen in the karyotypes prepared from higher ploidy metaphases.

Isoenzymes: AK-1, 1

ES-D, 1-2

G6PD, B

GLO-I, 1-2

Me-2, 1

PGM1, 1-2

PGM3, 1-2

Age: 63 years

Gender: male

Ethnicity: Caucasian

Comments: A contamination with Mycoplasma orale was cured in October 1971.

Propagation: RT4細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007

recommended serum:ATCC 30-2020

References: 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.

22365: O'Toole C, et al. Cellular immunity to human urinary bladder carcinoma. I. Correlation to clinical stage and radiotherapy. Int. J. Cancer 10: 77-91, 1972. PubMed: 4196436

22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080

22542: Lasfargues EY, et al. Isolation of two human tumor epithelial cell lines from solid breast carcinomas. J. Natl. Cancer Inst. 61: 967-978, 1978. PubMed: 212572

22660: Lasfargues EY, et al. A human breast tumor cell line (BT-474) that supports mouse mammary tumor virus replication. In Vitro 15: 723-729, 1979. PubMed: 94035

22723: Rigby CC, Franks LM. A human tissue culture cell line from a transitional cell tumour of the urinary bladder: growth, chromosone pattern and ultrastructure. Br. J. Cancer 24: 746-754, 1970. PubMed: 5503601

23226: Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212