產(chǎn)品描述
LXRE-Luc報(bào)告基因質(zhì)粒（LXRE luciferase reporter plasmid）是自主研發(fā)的用于檢測(cè)LXRE轉(zhuǎn)錄活性水平為目的的報(bào)告基因。LXR（liver X receptor）是核受體轉(zhuǎn)錄因子家族的成員之一，它與PPARs、FXR和RXR有著密切的關(guān)系。LXR對(duì)膽固醇、脂肪酸和葡萄糖穩(wěn)態(tài)有著重要的調(diào)節(jié)作用。
LXRE-Luc報(bào)告基因質(zhì)粒主要應(yīng)用于Liver X Receptor信號(hào)通路、**研究、相關(guān)基因的調(diào)控和功能的研究。
pGMLXRE-Lu是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)LXRE結(jié)合位點(diǎn)，可以高靈敏度地檢測(cè)LXRE的激活水平。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔儯黾恿速|(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外，由于質(zhì)粒體積減小，使得LXRE報(bào)告基因質(zhì)粒更易于轉(zhuǎn)染。LXRE Luciferase Reporter Plasmid LXRE-Luc報(bào)告基因質(zhì)粒
質(zhì)粒圖譜
LXRE Luciferase Reporter Plasmid LXRE-Luc報(bào)告基因質(zhì)粒運(yùn)輸與保存方法
冰袋（wet ice）運(yùn)輸。收到產(chǎn)品后放到-20 oC保存。
使用說明
pGMLXRE-Lu可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。用熒光素酶檢測(cè)試劑盒或雙熒光素酶檢測(cè)試劑盒進(jìn)行檢測(cè)。
注意事項(xiàng)
本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。LXRE Luciferase Reporter Plasmid LXRE-Luc報(bào)告基因質(zhì)粒
參考文獻(xiàn)
[1] Jin SH, et al. Resveratrol inhibits LXRα-dependent hepatic lipogenesis through novel antioxidant Sestrin2 gene induction.Toxicol Appl Pharmacol. 271(1):95-105 (2013).
[2] Inoue J, et al. Identification of MIG12 as a mediator for stimulation of lipogenesis by LXR activation. Mol Endocrinol. 25(6):995-1005 (2011).
[3] Cheng LC, et al. α-Lipoic acid ameliorates foam cell formation via liver X receptor α-dependent upregulation of ATP-binding cassette transporters A1 and G1.Free Radic Biol Med. 50(1):47-54 (2011).
rmal>[1] Rajkovic M, et al. Germ cell nuclear factor relieves cAMP-response element modulator tau-mediated activation of the testis-specific promoter of human mitochondrial glycerol-3-phosphate dehydrogenase. J Biol Chem. 279(50):52493-9 (2004).
[2] Greschik H, et al. Characterization of the DNA-binding and dimerization properties of the nuclear orphan receptor germ cell nuclear factor. Mol Cell Biol. 19(1): 690–703 (1999).LXRE Luciferase Reporter Plasmid LXRE-Luc報(bào)告基因質(zhì)粒
