產(chǎn)品描述
NF-κB-GFP報(bào)告基因是自主研發(fā)的用于檢測(cè)NFκB轉(zhuǎn)錄活性水平為目的的報(bào)告基因。 NF-κB基因在炎癥反應(yīng)、**應(yīng)答和抑制細(xì)胞凋亡等方面有著重要的作用。
NF-κB-GFP報(bào)告基因主要應(yīng)用于NF-κB信號(hào)通路的研究和基于NF-κB信號(hào)通路的**研究以及癌細(xì)胞中NF-KB的調(diào)控機(jī)制的研究。
pGMNF-ΚB-GFP是改造后的哺乳動(dòng)物真核表達(dá)載體，在其多克隆位點(diǎn)插入了多個(gè)NF-κB結(jié)合位點(diǎn)，可以高效地檢測(cè)NF-κB的激活水平。由于載體采用了GFP作為報(bào)告基因，更便于后續(xù)的檢測(cè)。同時(shí)，對(duì)載體中預(yù)測(cè)出的其它轉(zhuǎn)錄因子以外的結(jié)合位點(diǎn)進(jìn)行了適當(dāng)?shù)耐蛔?，增加了質(zhì)粒的轉(zhuǎn)錄因子結(jié)合特異性。另外，由于質(zhì)粒體積減小，使得NF-κB-GFP報(bào)告基因更易于轉(zhuǎn)染。NF-κB GFP Reporter Plasmid（NF-κB-GFP報(bào)告基因質(zhì)粒）
質(zhì)粒圖譜
NF-κB GFP Reporter Plasmid（NF-κB-GFP報(bào)告基因質(zhì)粒）pGMNF-κB -GFP質(zhì)粒測(cè)序引物
5’-TAGCAAAATAGGCTGTCCC-3’
運(yùn)輸與保存方法
冰袋運(yùn)輸。-20℃保存。
使用說(shuō)明
pGMNF-κB -GFP可以采用常規(guī)轉(zhuǎn)染方法轉(zhuǎn)染哺乳動(dòng)物細(xì)胞。
TNF-α、IL-1β和LPS等是常見(jiàn)的NF-κB激活劑，可作為NF-κB報(bào)告基因的陽(yáng)性對(duì)照。
注意事項(xiàng)
1）本質(zhì)粒未經(jīng)允許不得用于任何商業(yè)用途，也不得移交給訂貨人實(shí)驗(yàn)室以外的任何人或單位。
2）為了您的健康，實(shí)驗(yàn)操作時(shí)請(qǐng)穿實(shí)驗(yàn)服和帶一次性手套。NF-κB GFP Reporter Plasmid（NF-κB-GFP報(bào)告基因質(zhì)粒）
參考文獻(xiàn)
[1] Chang CT, et al. Comprehensive assessment of host responses to ionizing radiation by nuclear factor-κB bioluminescence imaging-guided transcriptomic analysis. PLoS One. 6(8):e23682(2011).
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[3] Barham W, et al. Intraductal injection of LPS as a mouse model of mastitis: signaling visualized via an NF-κB reporter transgenic.J Vis Exp. 2012 Sep 4;(67):e4030.
[4] Salerno KM, et al.TRAF family member-associated NF-kappa B activator (TANK) expression increases in injured sensory neurons and is transcriptionally regulated by Sox11.neuroscience. 11.034(2012).
[5] Nagasaki H, et al.Real-time monitoring of inflammation status in 3T3-L1 adipocytes possessing a secretory Gaussia luciferase gene under the control of nuclear factor-kappa B response element.Biochem Biophys Res Commun.420(3):623-7(2012).
[6] Wang DJ, et al.Regulation of the let-7a-3 promoter by NF-κB.PLoS One. 7(2):e31240(2012).
[7] Tran TA, et al.Lipopolysaccharide and tumor necrosis factor regulate Parkin expression via nuclear factor-kappa B.PLoS One. 6(8):e23660(2011).
[8] Robbins D, et al. NF-κB signaling in mice for screening anticancer drugs.Methods Mol Biol. 716:169-77(2011).
[9] Dvorák Z, et al. Differential effects of selected natural compounds with anti-inflammatory activity on the glucocorticoid receptor and NF-kappaB in HeLa cells.Chem Biol Interact. 159(2):117-28(2006).NF-κB GFP Reporter Plasmid（NF-κB-GFP報(bào)告基因質(zhì)粒）
[10] Ozeki M, et al. Response of cyclin B1 to ionizing radiation: regulation by NF-kappaB and mitochondrial antioxidant enzyme MnSOD.Anticancer Res. 24(5A):2657-63(2004).
