分子量 71.4kDa 價格 請咨詢當?shù)卮砩?/span>
純度 > 95%
來源 原核表達
Recombinant Peptidase Inhibitor 16 (PI16)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
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Organism species
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Homo sapiens (Human)
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Product No.
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HZQ943Hu01
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Source
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Prokaryotic expression
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Host
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E.coli
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Purity
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> 95%
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UOM
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50ug
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Predicted Molecular Mass
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71.4kDa
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Applications
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SDS-PAGE; WB; ELISA; IP.
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Endotoxin Level
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<1.0EU per 1μg (determined by the LAL method)
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Residues
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Leu28~Gly440 (Accession # Q6UXB8) with two N-terminal Tags, His-tag and GST-tag
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Formulation
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Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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SEQUENCES
肽酶抑制因子16(PI16)重組蛋白The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-LTD EEKRLMVELH NLYRAQVSPT ASDMLHMRWD EELAAFAKAY ARQCVWGHNK ERGRRGENLF AITDEGMDVP LAMEEWHHER EHYNLSAATC SPGQMCGHYT QVVWAKTERI GCGSHFCEKL QGVEETNIEL LVCNYEPPGN VKGKRPYQEG TPCSQCPSGY HCKNSLCEPI GSPEDAQDLP YLVTEAPSFR ATEASDSRKM GTPSSLATGI PAFLVTEVSG SLATKALPAV ETQAPTSLAT KDPPSMATEA PPCVTTEVPS ILAAHSLPSL DEEPVTFPKS THVPIPKSAD KVTDKTKVPS RSPENSLDPK MSLTGARELL PHAQEEAEAE AELPPSSEVL ASVFPAQDKP GELQATLDHT GHTSSKSLPN FPNTSATANA TGGRALALQS SLPGAEGPDK PSVVSGLNSG
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
肽酶抑制因子16(PI16)重組蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
肽酶抑制因子16(PI16)重組蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
