Lec8細(xì)胞

ATCC Number： CRL-1737?

細(xì)胞形態(tài)： 上皮樣

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

器官來源： 卵巢

運(yùn)輸方式： 凍存運(yùn)輸

Lec8細(xì)胞數(shù)量： 大量

生長狀態(tài)： 單層生長

Designations: Lec8 [originally named Pro-5WgaRVIII3D]

Depositors: P Stanley

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: monolayer

Organism: Cricetulus griseus

Morphology: epithelial

Source: Lec8細(xì)胞Organ: ovary

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Gender: female

Comments: This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin.

The cells exhibit a drastic reduction in the transport of UDP-galactose into the Golgi compartment, and may be useful for studying the role of galactose residues in the function and compartmentalization of glycosylated macromolecules.

The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).

The population contains Pro+ revertants at high frequency (approximately 1 in 250 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies.

These cells may also be grown as a suspension culture if agitated. Keep the cell concentration between 2 x 104 and 1 x 106 cells/ml.

Propagation: Lec8細(xì)胞ATCC complete growth medium: Alpha minimum essential medium, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, let sit for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

References: 1202: Stanley P. Selection of specific wheat germ agglutinin-resistant (WgaR) phenotypes from Chinese hamster ovary cell populations containing numerous lecR genotypes. Mol. Cell. Biol. 1: 687-696, 1981. PubMed: 9279382

22486: Deutscher SL, Hirschberg CB. Lec8細(xì)胞Mechanism of galactosylation in the Golgi apparatus. A Chinese hamster ovary cell mutant deficient in translocation of UDP-galactose across Golgi vesicle membranes. J. Biol. Chem. 261: 96-100, 1986. PubMed: 3510203