1F5細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2a

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： HB-9645?

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

1F5細(xì)胞Designations: 1F5

Depositors: Bristol-Myers Co.

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: 1F5細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human CD20 B cell antigen (Bp35)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The antibody is specific for CD20 (expressed on normal and neoplastic human B cells) and can induce proliferation of resting B cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

1F5細(xì)胞Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 21956: Senter PD, et al. Antibody-enzyme conjugates in combination with prodrugs for the delivery of cytotoxic agents to tumor cells. US Patent 4,975,278 dated Dec 4 1990

23287: Clark EA, et al. 1F5細(xì)胞Role of the Bp35 cell surface polypeptide in human B-cell activation. Proc. Natl. Acad. Sci. USA 82: 1766-1770, 1985. PubMed: 3872456